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一般山颗粒（SMD）ELISA套件 细节
Specification
●Sensitivity: 0.39ng/mL.
●Detection Range: 0.78-200ng/mL
●Specificity: This kit recognizes spermidine in samples.
No significant cross-reactivity or interference between spermidine and analogues was observed.
●Repeatability: Coefficient of variation is < 10%.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to
spermidine has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin
labeled spermidine and unlabeled spermidine (Standards or samples) with the pre-coated antibody specific to
spermidine. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish
Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse
proportional to the concentration of spermidine in the sample. After addition of the substrate solution, the intensity
of color developed is reverse proportional to the concentration of spermidine in the sample. Wuhan feiyuebio technology CO.,LTD
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Kit components & Storage
All the reagents should be kept according to the following conditions once the kit is received.
Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution. The volume of
reagents in partial shipments is a little more than the volume marked on the label, please use accurate
measuring equipment instead of directly pouring into the vial(s).
Other supplies required
Microplate reader with 450nm wavelength filter
High-precision transfer pipette, EP tubes and disposable pipette tips
Incubator capable of maintaining 37℃
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer
Lifes cience research test Kits
Antibodies - ELISA - Protein
Wuhan Feiyue Biotechnology Co., LTD., is a professional supplier of biological research reagents for life science community, mainly produce and export ELISA kit, protein, antibody.
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关于 武汉飞艺生物科技有限公司
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制造商/工厂
国家/地区
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主要产品
Elisa Kits...
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关于 武汉飞艺生物科技有限公司
FeiyueBio为生命科学和其他研究行业提供产品和解决方案，包括：ELISA Kits-抗体 - 蛋白质。我们将成为生物区域的好合伙人，拥有十多年的经验。 *******@*****.com电话：************** www.feiyuebio.com
