该供应商的新产品
环球卵形（OVA）ELISA套件 细节
Principle of the Procedure
This kit uses the Competitive-ELISA principle. The microtiter plate strips has been pre-coated with Coating
antibody for OVA. Standards or samples containing OVA and biotin labeled OVA are added to the plate, A
competitive inhibition reaction is launched between labeled OVA and free OVA (Standards or samples) with the
solid phase antibody specific to OVA. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a
sandwich complex of solid phase antigen-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is
added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter,
stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a
wavelength of 450nm. The concentration of OVA in the samples is then calculated from the OD value by
establishing a standard curve.Wuhan Feiyue Biotechnology Co.,Ltd
Email: [email protected]
Tel: 86-027-87002654
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Limitations of the Procedure
1. This kit is for laboratory scientific research only, we will not be responsible for any consequences if this kit is
used for clinical diagnosis or any other procedures.
2. Due to the uncertainty of its validity, this kit may not be suitable for testing some special experimental samples,
such as gene knockout experiments.
3. This kit should be used before its expiration date, and please strictly follow the instructions for storage.
4. Different manufacturers' kits or testing the same analyte by other methods may produce inconsistent results
because we do not compare our products with those of other manufacturers.
5. Since the antibodies used in the kit are usually prepared from recombinant proteins as immunogen, and
recombinant proteins can be limited by different fragmentation, expression and purification systems, it is not
recommended to use this kit to detect recombinant proteins
6. In order to get the best experimental results, please use only the reagents provided by the manufacturer, and
do not mix reagents from different batches.
7. Due to the existing conditions and the limitations of science and technology, we cannot fully identify and
analyze the raw materials provided by the supplier comprehensively. Therefore, the kit may have some quality
and technical risks.
8. The possibility of interference cannot be excluded before all factors are tested in the ELISA immunoassay.
9. In order to obtain reproducible results, each step in the experiment should be controlled and variations in
sample collection, handling and storage may also lead to differences in sample measurements.
10.Although each kit passes rigorous quality testing, differences in measured values between batches of kits can
still be caused by factors such as shipping conditions and different laboratory equipment.
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Storage & Stability
1. After receiving the kit, an unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month,
store each component separately at the temperature indicated in the table above once the kit is received.
2. when the kit is used, the remaining reagents should be stored according to the table above storage condition.
please return the unused strips to the aluminum foil bags containing the desiccant, and zip-seal the foil.
3. All reagent bottle caps must be tightened to prevent evaporation and microbial pollution, the volume of
imeasuring equipment instead of directly pouring into the vials.
4. The expiration date of the product is determined by the label on the box, and all components are guaranteed to
be stable during the shelf life.
Materials & Equipment Required But Not Provided
Microplate reader with 450nm wavelength filter
Incubator capable of maintaining 37℃
Single or multi-channel pipettes with high precision
disposable pipette tips
EP tubes
Container for Wash Solution
Squirt bottle, manifold dispenser, or automated microplate washer
Deionized or distilled water
Absorbent paper for blotting the microplate
关于 武汉飞艺生物科技有限公司
业务类型:
制造商/工厂
国家/地区:
Hubei, 中国
主要产品: Selling Elis Research Kit...
年总收入:
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成立时间:
不提供
业务性质
制造商/工厂
国家/地区
武汉, 中国
主要产品
Selling Elis Research Kit...
付款条件
Bank wire (T/T)
关于 武汉飞艺生物科技有限公司
武汉飞宇生物科技有限公司，位于武汉东湖新技术开发区，注册资金1500万元。专注于生物研究试剂和初级医疗设备的研发，生产，销售和进出口。 Feiyue Group自2005年以来一直在生物科技领域，拥有丰富的医疗器械研发，生产和销售经验。我们拥有自己的研究和销售团队，具有来自附属大学的专业技术支持，担任技术顾问。作为ELISA试剂盒，抗体，蛋白质，细胞和相关试剂的制造商，我们的目标是为研究人员提供最佳产品和相关的定制服务，以便他们可以对其项目具有良好的启动。通过特殊的技术支持得到了高质量的保证。 *@********************
